ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of berbamine on human hepatoma cell line SMMC7721.</p><p><b>METHODS</b>The effects of 24 h and 48 h incubation with different concentrations (0 to approximately 64 microg/ml) of the berbamine on SMMC7721 cells were evaluated using 3-4,5-dimethylthiazol-2-yl-2,5-diphenyltetrazolium bromide (MTT) assay. Hoechst 33258 staining was conducted to distinguish the apoptotic cell, and the appearance of sub-G1 stage was determined by PI (propidium iodide) staining, the percentage of apoptotic cell was determined by flow cytometry following annexin V/PI staining. Flow cytometry was performed to analyze the cell cycle distribution and the mitochondrial membrane potential (psi(m)); the expression of activated caspase3 and caspase9 was analyzed by Western-blot.</p><p><b>RESULTS</b>The proliferation of SMMC7721 was decreased after treatment with berbamine in a dose- and time-dependent manner. Berbamine could induce apoptosis in SMMC7721 cells and could cause cell cycle arrest in G0/G1 phase, to induce loss of mitochondrial membrane potential (psi(m)) and activate caspase3 and caspase9. Berbamine-induced apoptosis could be blocked by the broad caspase inhibitor z-VAD-fmk.</p><p><b>CONCLUSION</b>Berbamine exerts antiproliferative effects on human hepatocellular carcinoma SMMC7721 cells. The anticancer activity of berbamine could be attributed partly to its inhibition of cell proliferation and induction of apoptosis in cancer cells through loss in mitochondrial transmembrane potential and caspase activation.</p>
Subject(s)
Humans , Alkaloids , Pharmacology , Apoptosis , Benzylisoquinolines , Pharmacology , Carcinoma, Hepatocellular , Metabolism , Pathology , Caspase 3 , Metabolism , Caspase 9 , Metabolism , Cell Line, Tumor , Enzyme Activation , Liver Neoplasms , Metabolism , Pathology , Medicine, Chinese Traditional , Membrane Potentials , Mitochondria, Liver , MetabolismABSTRACT
We identified a novel gene ST13 from a subtractive cDNA library of normal intestinal mucosa in 1993, more studies showed that ST13 was a co-chaperone of Hsp70s. Recently we detected the ST13 gene expression in tumor tissue and adjacent normal tissue of the same colorectal cancer patient and investigated if the ST13 gene expression might have any prognostic value. Analysis was performed at molecular level by reverse transcription-PCR using real-time detection method. We measured two genes simultaneously, ST13 as the target gene and glyceraldehydes-3-phosphate dehydrogenase as a reference gene, in primary colorectal tumor specimens and tumor-adjacent normal mucosa specimens from 50 colorectal cancer patients. The expression levels of the ST13 gene were significantly decreased in primary tumors compared with adjacent mucosa (P<0.05). But there were no significant differences in the expression of ST13 as compared with different Dukes' stage, tumor differentiation grade, invasion depth, lymph node metastasis and disease-specific survival.
Subject(s)
Female , Humans , Male , Biomarkers, Tumor , Metabolism , Carrier Proteins , Metabolism , China , Epidemiology , Colorectal Neoplasms , Diagnosis , Metabolism , Mortality , Disease-Free Survival , Gene Expression Profiling , Prevalence , Prognosis , Risk Assessment , Methods , Risk Factors , Survival Analysis , Survival Rate , Tumor Suppressor Proteins , MetabolismABSTRACT
<p><b>OBJECTIVE</b>To study the effect of Kanglaite injection on cyclooxygenase activity in lung carcinoma A549 cells.</p><p><b>METHODS</b>The expression of mRNA of COX-1 and COX-2 were measured by RT-PCR. The expression of COX-2 protein was measured by Western-blot.</p><p><b>RESULT</b>Kanglaite injection could selectively decreased COX-2 mRNA expression and protein expression while COX-1 mRNA expression was unchanged.</p><p><b>CONCLUSION</b>Kanglaite injection is selectively inhibitory agent of COX-2, and possess inhibitory effects on cyclooxygenase 2.</p>
Subject(s)
Humans , Antineoplastic Agents, Phytogenic , Pharmacology , Carcinoma, Non-Small-Cell Lung , Pathology , Cell Line, Tumor , Coix , Chemistry , Cyclooxygenase 1 , Genetics , Cyclooxygenase 2 , Genetics , Drugs, Chinese Herbal , Pharmacology , Gene Expression Regulation, Neoplastic , Injections , Lung Neoplasms , Pathology , Plant Oils , Pharmacology , Plants, Medicinal , Chemistry , RNA, Messenger , Genetics , Reverse Transcriptase Polymerase Chain Reaction , Seeds , ChemistryABSTRACT
<p><b>OBJECTIVE</b>To study the effect and mechanism of berbamine on the apoptosis of multidrug resistant leukemia K562/Adr cells and in reversing the drug resistance.</p><p><b>METHODS</b>IC50 value of K562/Adr cell was determined with MTT method, cell apoptosis rate was analyzed by flow cytometry with Annexin V FITC-PI assay, with the peak and cell cycle detected by PI staining. At the same time, flow cytometry was also used in determining Caspase-3, P-GP protein expression and drug accumulating capacity in cells, and RT-PCR method was used to analyze the gene expression of mdr-1.</p><p><b>RESULTS</b>Berbamine could inhibit human leukemia K562/Adr cell growth in dose-dependent manner, it could also induce cell apoptosis, increase the protein expression of Caspase-3 and the drug excretion capacity of cells, reduce the mRNA and protein expression levels of mdr-1 gene.</p><p><b>CONCLUSION</b>Berbamine could activate Caspase-3 to induce human leukemia K562/Adr cell apoptosis, and by reducing mdr-1 gene expression to reverse its multidrug resistance.</p>
Subject(s)
Humans , ATP Binding Cassette Transporter, Subfamily B, Member 1 , Genetics , Alkaloids , Pharmacology , Antineoplastic Agents, Phytogenic , Pharmacology , Apoptosis , Benzylisoquinolines , Pharmacology , Caspase 3 , Caspases , Genetics , Drug Resistance, Neoplasm , Genetics , K562 Cells , RNA, Messenger , GeneticsABSTRACT
<p><b>OBJECTIVE</b>To summarize the clinical experience from treatment of patients with severe acute respiratory syndrome (SARS).</p><p><b>METHODS</b>Retrospective analysis of seven patients with SARS in Ditan hospital treated since April 22 in 2004 was performed.</p><p><b>RESULTS</b>In the 7 patients, 2 were male, 5 were female, and the average age was (35.3 plus/minus 11.3) years. The main clinical manifestations were fever, cough, minor or serious dyspnea, nausea, signs of injury to other organs, and so on. The treatment regiments included oxygen, small dosage and short period of methylprednisolone (1 to 2 mg/kg), use of ventilator, psychological intervention, and treatment of underlying diseases, after which, all the 7 patients recovered.</p><p><b>CONCLUSION</b>Rational use of methylprednisolone and timely use of ventilator were the key steps of treatment.</p>
Subject(s)
Adult , Female , Humans , Male , Middle Aged , Anti-Inflammatory Agents , Therapeutic Uses , Combined Modality Therapy , Cross Infection , Drug Therapy , Therapeutics , Infectious Disease Transmission, Patient-to-Professional , Methylprednisolone , Therapeutic Uses , Oxygen Inhalation Therapy , Retrospective Studies , Severe Acute Respiratory Syndrome , Therapeutics , Ventilators, MechanicalABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of sodium hyaluronate on the growth and adhesion of colorectal cancer cells.</p><p><b>METHODS</b>Human colorectal cancer cell lines SW620 and Colo205 were treated with sodium hyaluronate (25 -2,500 microg/ml), and cancer cell proliferation was measured by MTT assay in vitro. Flow-cytometric analysis was applied to detect expression of CD44 on SW620 and Colo205 cells.</p><p><b>RESULT</b>In vitro sodium hyaluronate enhanced proliferation of Colo205 cells, but it had no appreciable effect on SW620 growth under the same doses, Meantime, CD44 expression on cancer cells decreased compared with controls.</p><p><b>CONCLUSION</b>In vitro sodium hyaluronate has different effects on growth of different colorectal cancer cell lines, but can inhibit CD44 expression of colorectal cancer cells and influence their ability of adhesion.</p>
Subject(s)
Humans , Cell Adhesion , Cell Division , Cell Line, Tumor , Colorectal Neoplasms , Pathology , Hyaluronan Receptors , Hyaluronic Acid , PharmacologyABSTRACT
<p><b>OBJECTIVE</b>To present a batch of data of transected pancreatic neck injuries and to sum up the experience in surgical interventions for the injuries.</p><p><b>METHODS</b>We analysed 13 patients with a transected injury to the pancreatic neck from Jan. 1995 to Dec. 2000. External drainage was performed in all patients. Pancreatoduodenectomy was conducted in 2 patients with a transected injury to the pancreatic neck associated with duodenal ruptures, and TPN was administered immediately after operation. Proximal closure of the transected margin and distal pancreaticojejunostomy was performed in 4 patients. Proximal closure of the transected margin and distal pancreaticojejunostomy plus splenectomy was performed in 7 patients associated with contusion of pancreatic body or tail plus spleen rupture.</p><p><b>RESULTS</b>12 patients healed and one patient died of anesthetic accident during the course of restoration of the dislocation of his right hip joint. Complications occurred in 7 patients.</p><p><b>CONCLUSIONS</b>The operation should be performed according to the degree of the injuries and associated duodenal injuries. Routine drainage and nutrient support should be recommended.</p>
Subject(s)
Adolescent , Adult , Child , Female , Humans , Male , Middle Aged , Drainage , Nutritional Support , Pancreas , Wounds and Injuries , General SurgeryABSTRACT
<p><b>AIM</b>To study the antitumor effect of baicalein on human leukemia K562 cell and its mechanism.</p><p><b>METHODS</b>The IC50 value and cytotoxity of K562 cell were detected by MTT method. The apoptotic cell was analyzed by FCM using Annexin V FITC--PI staining method. Sub-G1 peak was also measured by FCM. Protein expressions of Bcl-2, Fas, Caspase 3 were evaluated with FCM.</p><p><b>RESULTS</b>Baicalein was shown to significantly inhibit the proliferation of K562 cell in a dose-dependent manner and selectively induce apoptosis of human leukemia K562 cells. Flow cytometric analysis showed that baicalein arrested K562 cells in the S phase. In addition, protein expression of Fas, Caspase 3 of K562 cells increased after exposure to baicalein, but Bcl-2 was unchanged.</p><p><b>CONCLUSION</b>Baicalein can selectively induce apoptosis of human leukemia K562 cell dose and time dependently through up-regulation of caspase-3 and fas gene expression level.</p>